Location
- Research Support Building (CACTUS)
- Rúa de Constantino Candeira, 1. Campus Vida , 15782Santiago de Compostela
- Phones
- 881 816 242
Identification of proteins by fragmentation spectra of some of the peptide masses obtained, using the Ultraflex III TOF/TOF mass spectrometer (Bruker) and their correlation with existing databases. Fragmentation of the peptides can be carried out by LID fragmentation (metastable decomposition) or CID Collision-Induced Dissociation (high energy fragmentation).
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A process of reduction and carbamidomethylation from proteins in solution, or in gel, followed by enzymatic digestion with trypsin (an enzyme that specifically breaks down lysines and arginines, if not followed by a proline), is carried out to obtain their peptides.
The tryptic digest is analysed using the MALDI-TOF technique from which the m/z ratio of the peptides generated in the digestion is obtained, and fragmentation of some of the masses of the peptides obtained. These experimental masses will be compared with the theoretical masses in the databases and their correlation will lead to an identification, using a search engine (PEAKS, MASCOT).
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Mass Spectrometry and Proteomics Unit
- Research Support Building (CACTUS)
- Rúa de Constantino Candeira, 1. Campus Vida , 15782Santiago de Compostela
- 881 816 242
