Our expertise
Our assays
Our technologies
Our Rates
Localization and contact
Our expertise
Drug discovery program design
- Candidate Target Profile definition
- Feasibility/druggability assessment
- Screening cascade design
- Identification of hits, leads and candidates
Assay development, miniaturization, and automation
- Target-based assays
- Phenotypic assays
- Deconvolution assays
- Custom assays
De-risking programs
- Drug discovery programs up to proof of concept stage
Chemical compound libraries
- Compounds selected in silico based on biological and structural diversity
- Proprietary compounds
- Drug repurposing libraries
- Target-focused sub-libraries
Pharmacogenomics studies
- Biomarkers identification
- Identification of novel therapeutic targets
In vitro preliminary ADME-Tox and safety
- Cytochrome P450 induction and inhibition
- Cytotoxicity
- hERG channel
- Chemical, microsomal and plasma stability
- Plasma protein binding
- Permeability assay
- P-glycoprotein
- Genotoxicity
Customized assay development
- Target engagement
- Phenotypic assays
- Deconvolution assays
Our assays
From INNOPHARMA we are constantly developing new tests and implementing new technologies, so if you have any interest in any test or technology not included in this list, please contact us
COVID-19
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ACE2 activity
TMPRSS2 activity
Furin activity |
TARGET-BASED
Radioligand Assays |
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Adenosine |
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A1
A2A
A2B
A3 |
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Dopamine |
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D1
D2
D3
D4
D5 |
Histamine |
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H1
H2
H3 |
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Adrenergic: |
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α1
α2
α2C
β1
β2
β3 |
Serotonin |
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5-HT1A 5-HT1B 5-HT1D
5-HT2A 5-HT2B 5- HT2C
5-HT5A 5-HT6 5-HT7 |
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Cannabinoid: |
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CB1
CB2 |
Muscarinic: |
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M1
M2
M3
M4
M5 |
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Second Messengers |
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Arachidonic acid metabolism |
Nitric oxide synthase |
Inositol phosphate |
Calcium mobilization |
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Isolated Organ Assays |
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Adenosine: A1 A2A A2B
Adrenergic: α1 α2 β1
Histamine: H1 H2
Muscarinic: M1 M2 M3
Serotonin: 5-HT2A 5-HT2B 5-HT3 5-HT4 |
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Beta-Arrestin Translocation - BRET |
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Radioligand Assays |
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Dopamine (DAT) |
Noradrenaline (NAT) |
Serotonin (5-HTT) |
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Radioligand Assays |
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Estrogen receptors (ER)
Androgen receptor (AR)
Progesterone receptor (PR)
Glucocorticoid receptor (GR)
Peroxisome proliferator-activated receptors (PPAR)
Prostanoid receptors (PR) |
Functional assays |
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Androgen receptor (AR)
Glucocorticoid receptor (GR) |
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Kinase profiling > 250 functional assays |
Phosphodiesterases: 15 activity assays |
Phosphatases |
Beta-secretase |
Acetylcholinesterase |
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Monoamine oxidases: MAO-A, MAO-B |
Epigenetic enzymes |
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Voltage-gated |
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Calcium
Potasium
Sodium
hERG |
Membrane ligand-gated |
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NMDA
GABAA
5-HT3 |
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PHENOTYPIC ASSAYS
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CELL VIABILITY AND CYTOTOXICITY IN 20+ HUMAN CELL LINES
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MORPHOLOGICAL PROFILING BY HIGH-CONTENT IMAGING (CELL PAINTING)
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PHENOTYPIC PROFILING BY LABEL-FREE DYNAMIC MASS REDISTRIBUTION-BASED ASSAYS
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TARGET-DECONVOLUTION STRATEGIES
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IN VITRO TRANSLATIONAL DISEASE-RELATED ASSAYS
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Neurological and neurodegenerative diseases |
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Neurite outgrowth
α-synuclein aggregation |
Metabolic- disease |
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Adipocyte assay |
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PRELIMINARY ADME-Tox AND SAFETY ASSAYS
Cell Lines |
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Cancer cell lines |
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Human ovarian carcinoma: A2780, A2780cis, NCI-ADR/RES
Human cervix carcinoma: HeLa 229
Human breast carcinoma: MCF-7, T47D, MDA-MB-231, Hs578T
Human lung carcinoma: NCI-H460, A549
Human glioblastoma: SF268
Human colon carcinoma: CACO-2, HT-29, HCT116
Human liver carcinoma: Hep-G2
Human neuroblastoma: SH-SY5Y
Human acute myeloid leukemia: HL-60
Human pancreatic carcinoma: CFPAC-1
Human prostate carcinoma: LNCaP, PC3
Human osteosarcoma: U2OS |
Other cell lines |
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Human lung fibroblasts: MRC-5
Porcine kidney cell line: LLC-PK1 |
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Cell Viability Technologies |
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Crystal violet |
MTT reduction |
Sulforhodamine B |
ATP production |
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CYTOCHROME INDUCTION AND INHIBITION
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Recombinant P450 (1A2, 2C9, 2C19, 2D6, 3A4) |
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CHANNEL PERMEABILITY ASSAYS
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CACO-2 (6-well, 24-well and 96-well formats) |
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Aqueous solubility (pH-dependent) |
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CUSTOMIZED ASSAYS
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AD-HOC ASSAY DEVELOPMENT AND SCREENING
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Target engagement |
Phenotypic assays |
Target deconvolution strategies |
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Our technologies
The Innopharma Platform has state-of the art equipment for carrying out assay development projects and screening campaigns with different technological approaches
- Radioactivity (Filtration and SPA)
- Absorbance
- Fluorescence intensity and polarization
- Luminescence
- FRET and Homogeneous Time-Resolved Fluorescence
- BRET
- Alphascreen
- Fluorescence Lifetime Imaging
- Automated patch-clamp
- Label free (Dynamic Mass Redistribution)
- Automated mobility shift (Lab on a chip)
- High Content Screening
- UPLC-MS/MS
- Genome wide and candidate gene association studies
- Next Generation Sequencing
Our Rates
Click to see your rates
Localization and contact
- Molecular Medicine and Chronic Diseases Research Center (CIMUS)
Avenida de Barcelona, s/n, 15782
Santiago de Compostela
- 881 815 459
- innopharma [at] usc.es
The contents of this page were updated on 10.02.2024.