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    Research infrastructures

    Low resolution - ESI/APCI/APPI - FIA

    Location

    Research Support Building (CACTUS)
    Rúa de Constantino Candeira, 1. Campus Vida , 15782
    Santiago de Compostela
    Phones
    881 816 242
    • Operational equipment
    This is a flow injection analysis (FIA) technique, where HPLC is used without a chromatographic column, so samples must be of high purity. The default mobile phase used is a 1:1 H2O/MeOH mixture acidified with 0.1 % formic acid.

      The polarity of the sample has to be taken into account in order to choose the appropriate analytical technique. ESI is suitable for analytes of medium-high polarity that dissolve in polar solvents such as water or methanol. APCI is suitable for analytes of low-medium polarity that dissolve in non-polar solvents such as dichloromethane or chloroform; and APPI is suitable for samples that have chromophore groups.

      Samples should be delivered in identified and labelled vials or tubes, with a minimum user-supplied quantity of 0.1 mg, indicating suitable solvents (if possible, solvents such as DMF or DMSO should not be used). If they are in solution, the solvent used should be indicated. In addition, the presence of salts may interfere with sample acquisition and results. In this case, a separate desalting can be performed with Zip-Tip Desalting (chargeable service).

      The instrument used has interchangeable ESI, APCI and APPI ionisation sources and, in addition, as it is an ion trap, it is possible to obtain the spectrum in positive and negative mode in the same measurement, in a range of 50 to 3000 m/z.

    • Mass Spectrometry and Proteomics Unit

      Research Support Building (CACTUS)
      • Rúa de Constantino Candeira, 1. Campus Vida , 15782
        Santiago de Compostela
      • 881 816 242