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    Research infrastructures

    High resolution - nanoESI

    Location

    Research Support Building (CACTUS)
    Rúa de Constantino Candeira, 1. Campus Vida , 15782
    Santiago de Compostela
    Phones
    881 816 242
    • Operational equipment
    NanoESI employs the same technique as ESI, with the difference being the flow rate used in the introduction of the dissolved analyte into the ionisation source. While ESI works with flow rates of mL/min or µL/min, nanoESI works with flow rates of nL/min. In addition, the capillary is smaller in diameter than the one used in the ESI process. Thus, the sensitivity of the process is increased instead of increasing the analyte concentration in the sample.

    • Samples for the ESI technique must have a concentration required for analysis, summarised in this table:

      Concentration required for ESI analysis

      MW (Da) 

      µg/ml 

      500  

      10-25  

      1,000  

      20-50  

      2,500  

      50-130  

      5,000  

      100-250  

      This technique is suitable for analytes with very low concentration, due to the increased sensitivity that comes with the use of nanoflows, with a 1000-time lower sample requirement.

      This technique is recommended for analytes of medium-high polarity, when they are found in a very low concentration or quantity. Among these molecules we could point out, among others, organic and inorganic compounds, carbohydrates, proteins, peptides, etc.

      In the case of samples with concentrations below those indicated in the table, analysis with the nanoESI source can be carried out in specific cases, after consultation.

    • Mass Spectrometry and Proteomics Unit

      Research Support Building (CACTUS)
      • Rúa de Constantino Candeira, 1. Campus Vida , 15782
        Santiago de Compostela
      • 881 816 242