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    Research infrastructures

    Leica TCS-SP2

    Location

    Research Support Building (CACTUS)
    Rúa de Constantino Candeira, 1. Campus Vida , 15782
    Santiago de Compostela
    Schedule
    Monday to Friday, from 10 a.m. to 2 p.m. and from 4 p.m. to 6 p.m.
    • Operational equipment
    Microscope with confocal and multi-photon spectral system.
      • Excitation lines: 405nm, 458nm, 476nm, 514nm, 561nm and 633nm.
      • Spectral detection range: 440-850nm.
      • Simultaneous acquisition of up to 3 detection channels for fluorescence and reflection, and one channel for transmitted illumination.
      • Advanced Time-Lapse series acquisition for FRET and FRAP applications.
      • Image processing:
        • Three-dimensional reconstruction.
        • 2D and 3D co-localisation analysis.
        • Physiological measurements.
        • A posteriori separation of fluorochromes with overlapping emission spectra.
        • Reconstruction of surfaces, topographical measurements and roughness parameters (according to DIN EN ISO 4287).
      • Possibility of working in epifluorescence mode with image capture with the Leica DFC 350 FX camera.

    • Operating modes

      • Fluorescence (3 channels), reflection and transmission (interferential contrast).
      • Acquisition of multidimensional series: x, y, z, t, l (area, height, tempo, rotation and wavelength).
      • Scanning of ROI Scan regions of interest with the possibility of using independent excitation wavelengths in each ROI.

      Excitation range with 7 laser lines

      • Blue Diode (405nm).
      • Argon laser (458nm, 476nm, 488nm, 514nm).
      • DPS diode (561 nm).
      • Helium-Neon laser (633 nm).

      Spectral detection range: 400-850 nm.

      Maximum resolution: 4,096 x 4,096 pixels.

      Magnification (lenses):

      • HC PL FLUOTAR 10x/0.3 Dry.
      • HC PL APO CS10x/0.4 Dry.
      • HC PL FLUOTAR 20x/0.5 Dry.
      • HC PL APO CS 20x/0.7 Dry.
      • HC PL FLUOTAR 40x/1.0 Oil.
      • HCX PL CS 40x/1.25 Oil.
      • HCX PL CS 63x/1.32 Oil.
      • HCX PL Ibd. BL 63x/1.40 Oil.
      • HCX PL APO 63x/1.30 Glyc.
      • HCX L 63x/0.9 Water.
      • HCX PL FLUOTAR 100x/1.30 Oil
      • HCX PL CS 100x/1.40 Oil

      Epifluorescence imaging on the upright and inverted microscopes with the Leica DFC 350 FX camera.

      Filters:

      • A UV BP 340-380 excitation, LP 425 emission.
      • I3 Blue excitation BP 450-490, LP 515 emission.
      • N2.1 Green excitation BP 515-560, LP590 emission.

    • Electronic and Confocal Microscopy and Support for Biological Specialities Unit

      Research Support Building (CACTUS)
      • Rúa de Constantino Candeira, 1. Campus Vida , 15782
        Santiago de Compostela

      Electronic and Confocal Microscopy and Support for Biological Specialities Unit

      Research Support Building (CACTUS). Lugo
      • Campus Lugo s/n, 27002
        Lugo