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Research infrastructures

Leica TCS-SP2

Location

Research Support Building (CACTUS)
Rúa de Constantino Candeira, 1. Campus Vida , 15782
Santiago de Compostela
Schedule
Monday to Friday, from 10 a.m. to 2 p.m. and from 4 p.m. to 6 p.m.
  • Operational equipment
Microscope with confocal and multi-photon spectral system.
    • Excitation lines: 405nm, 458nm, 476nm, 514nm, 561nm and 633nm.
    • Spectral detection range: 440-850nm.
    • Simultaneous acquisition of up to 3 detection channels for fluorescence and reflection, and one channel for transmitted illumination.
    • Advanced Time-Lapse series acquisition for FRET and FRAP applications.
    • Image processing:
      • Three-dimensional reconstruction.
      • 2D and 3D co-localisation analysis.
      • Physiological measurements.
      • A posteriori separation of fluorochromes with overlapping emission spectra.
      • Reconstruction of surfaces, topographical measurements and roughness parameters (according to DIN EN ISO 4287).
    • Possibility of working in epifluorescence mode with image capture with the Leica DFC 350 FX camera.

  • Operating modes

    • Fluorescence (3 channels), reflection and transmission (interferential contrast).
    • Acquisition of multidimensional series: x, y, z, t, l (area, height, tempo, rotation and wavelength).
    • Scanning of ROI Scan regions of interest with the possibility of using independent excitation wavelengths in each ROI.

    Excitation range with 7 laser lines

    • Blue Diode (405nm).
    • Argon laser (458nm, 476nm, 488nm, 514nm).
    • DPS diode (561 nm).
    • Helium-Neon laser (633 nm).

    Spectral detection range: 400-850 nm.

    Maximum resolution: 4,096 x 4,096 pixels.

    Magnification (lenses):

    • HC PL FLUOTAR 10x/0.3 Dry.
    • HC PL APO CS10x/0.4 Dry.
    • HC PL FLUOTAR 20x/0.5 Dry.
    • HC PL APO CS 20x/0.7 Dry.
    • HC PL FLUOTAR 40x/1.0 Oil.
    • HCX PL CS 40x/1.25 Oil.
    • HCX PL CS 63x/1.32 Oil.
    • HCX PL Ibd. BL 63x/1.40 Oil.
    • HCX PL APO 63x/1.30 Glyc.
    • HCX L 63x/0.9 Water.
    • HCX PL FLUOTAR 100x/1.30 Oil
    • HCX PL CS 100x/1.40 Oil

    Epifluorescence imaging on the upright and inverted microscopes with the Leica DFC 350 FX camera.

    Filters:

    • A UV BP 340-380 excitation, LP 425 emission.
    • I3 Blue excitation BP 450-490, LP 515 emission.
    • N2.1 Green excitation BP 515-560, LP590 emission.

  • Electronic and Confocal Microscopy and Support for Biological Specialities Unit

    Research Support Building (CACTUS)
    • Rúa de Constantino Candeira, 1. Campus Vida , 15782
      Santiago de Compostela

    Electronic and Confocal Microscopy and Support for Biological Specialities Unit

    Research Support Building (CACTUS). Lugo
    • Campus Lugo s/n, 27002
      Lugo