ECTS credits ECTS credits: 4.5
ECTS Hours Rules/Memories Student's work ECTS: 74.2 Hours of tutorials: 2.25 Expository Class: 18 Interactive Classroom: 18 Total: 112.45
Use languages Spanish, Galician
Type: Ordinary Degree Subject RD 1393/2007 - 822/2021
Center Faculty of Biology
Call: Second Semester
Teaching: Sin Docencia (En Extinción)
Enrolment: No Matriculable (Sólo Planes en Extinción)
The goal is that learning outcome of this matter by alumni to enable them:
-Understand the concepts, terminology and basic methodology of genetic engineering and some of its applications.
-Manage the literature and present work related to the Genetic Engineering and Biotechnology.
SUBJECT WITHOUT TEACHING, ONLY WITH RIGHT TO EVALUATION
The contents of the last course with teaching are indicated, which will be evaluated this course.
PROGRAM AND DURATION OF LECTURES (FACE-TO-FACE) (26h)
LESSON 1. INTRODUCTION TO GENETIC ENGINEERING AND BIOTECHNOLOGY (1.5h)
LESSON 2. RECOMBINANT DNA ENZYMOLOGY. (3h)
LESSON 3. NUCLEIC ACID AND PROTEINS HYBRIDIZATION. (2h)
LESSON 4. DNA CLONING IN E. coli (3h)
LESSON 5. LIBRARIES AND MICROARRAYS. (1.5h)
LESSON 6. PCR. (2h)
LESSON 7. DNA SEQUENCING. (2h)
LESSON 8. DIRECTED SITE MUTAGENESIS AND PROTEIN ENGINEERING. (2h)
LESSON 9. EXPRESSION OF RECOMBINANT PROTEINS IN E. coli. (2.5h)
LESSON 10. GENETIC ENGINEERING WITH YEAST. (1.5h)
LESSON 11. DNA CLONING IN ANIMAL CELLS. (2h)
LESSON 12. BIOTECHNOLOGY IN ANIMALS AND BIOMEDICAL APPLICATIONS. (2h)
LESSON 13. BIOTECHNOLOGY IN PLANTS. (1h)
PROGRAM OF LABORATORY PRACTICE (FACE-TO-FACE) (5h)
-PRACTICE 1. DNA digestion with restriction enzymes. Purification of genomic DNA from saliva; PCR amplification of a region of the purified genome (2h 30 ').
-PRACTICE 2. Agarose gel analysis of DNA fragments of restriction and PCR products. Assessment of the results obtained in the two sections (2h 30’).
SEMINAR PROGRAM (FACE-TO-FACE) (5h)
-Seminar 1. Labeling techniques of hybridization probes (1h)
-Seminar 2. Variations of the basic technique of PCR and its applications (1h)
-Seminar 3. DNA sequencing techniques. (1h)
-Seminar 4. Realization of problems of the matter (2h)
PROGRAM OF THE SMALL GROUP TUTORING (5h)
-Tutorial 1. (Telematic) Presentation to students of the work of seminars to be made by each group throughout the course, detailed presentation of the teaching guide. (1h)
-Tutorials 2, 3, 4 and 5. (Face-to-face or telematics) Resolution of doubts and questions about the lectures, seminars and practices. They will be carried out at the request of the students. (1h each)
EXAM (2h)
Mandatory face-to-face written exam of the contents of lectures and seminars.
Basic
-Brown, T.A., 2016. Gene cloning and DNA analysis: An Introduction. 7th ed. Chichester: Wiley-Blackwell.
-Clark, D.P. and Pazdernik, N.J., 2016. Biotechnology. [on line] 2nd ed. Amsterdam: Elsevier Academic Press. Available in: https://www-sciencedirect-com.ezbusc.usc.gal/book/9780123850157/biotech…
-Perera, J., Tormo, A. y García, J.L., 2002. Ingeniería genética. 2 v. Madrid: Síntesis.
-Real García, M.D., Rausell Segarra, C. y Latorre Castillo, A., 2017. Técnicas de ingeniería genética. Madrid: Síntesis.
Complementary
-Clark, D.P, 2019. Molecular Biology. [on line] 3rd ed. Amsterdam: Elsevier . Available in: https://www-sciencedirect-com.ezbusc.usc.gal/book/9780128132883/molecul…
-Glick, B.R. and Patten, C.L., 2017. Molecular biotechnology: principles and applications of recombinant DNA. 5th ed. Washington DC: ASM Press.
-Green, M.R. and Sambrook, J., 2012. Molecular cloning: a laboratory manual. 4th ed. 3 v. Cold Spring Harbor: Cold Spring Harbor Laboratory Press.
-McPherson, M.J. and Møller, S., 2006. PCR. 2nd ed. New York: Taylor & Francis.
-Renneberg, R., Berkling, V., and Loroch, V., 2016. Biotechnology for beginners [on line]. Amsterdam: Elsevier. Available in: https://www-sciencedirect-com.ezbusc.usc.gal/book/9780128012246/biotech…
Another resources
- http://biomodel.uah.es. Web Universidad Alcalá de Henares
-https://www.jove.com/science-education-library. Vídeos educativos sobre diferentes técnicas de bioloxía molecular e enxeñaría xenética.
- http://www.ncbi.nlm.nih.gov/guide/. The National Center for Biotechnology Information, (NCBI) main source of databases (nucleotides, genes and genomes) and bioinformatics tools.
-Electronic resources of BUSC: Cell, Nature, Science, Sciencedirect, etc.
The subject of Genetic Engineering participates, in greater or lesser extent, in the acquisition by the student of the basic and general competencies that appear in the Biology Degree Report.
The specific skills that develop in this area, as recounted in the notes grade in Biology are as follows:
CE1.c - Sphere of competence of instrumental knowledge, skills and abilities:
-Basic techniques in biology
CE2.e - Sphere of competence of origin and molecular basis of life:
-Analysis of biomolecules and metabolic processes.
CE2.f - Sphere of competence of origin and molecular basis of life:
-Molecular, cellular and tissue analysis,
CE7.d - Sphere of competence of biotechnology and biomedicine:
- Biotechnology and recombinant DNA.
CE7.g Sphere of competence of biotechnology and biomedicine:
-Methods of clinical and molecular analysis.
CE7.h - Biotechnology and recombinant DNA.
-Agri-food and forestry applications.
The subject of Genetic Engineering participates, in greater or lesser extent, in the acquisition by the student of the following transversal competences:
CT1 - Ability to analyze and synthesize.
CT2 - Ability to reason and argument.
CT3 - Ability to work together and address problematic situations collectively.
CT4 - Ability to obtain adequate information, diverse and updated.
CT5 - Ability to develop and present an organized and understandable text.
CT6 - Ability to make a public display in a clear, concise and consistent.
CT7 - Commitment to accuracy of the information provided to others.
CT8 - Skill in the use of ICT.
CT9 - Use of bibliographic and Internet.
CT10 - Use information in foreign language.
CT11 - Ability to solve problems through the integrated application of knowledge.
THIS SUBJECT IS IN EXTINCTION AND WILL NOT HAVE TEACHING.
CONTENT DEVELOPMENT AND MANDATORY ATTENDANCE
Paragraph CONTENTS OF THE MATTER shows the approximate durations of the different activities of students in the classroom or laboratory.
a) Theory subject program will be presented by Professor lecture format in the lectures, which are not compulsory for students. Will last approximately 1hour. In the lectures, in addition to waxing, it will use audio-visual presentations, which will be accessible from the virtual course, to make it easier to track the contents. In classes, students can disrupt the teacher's explanations with questions or concerns, as well as the teacher can ask questions and request participation of learners in discussion and presentation of content and require finding information for delivery in the next class. Lectures require daily follow-up and dedication (in the classroom and outside it) because the teacher will present the basics of the subject that must be deepened through the management of the literature and study. It will work, in general, all the powers and objectives of subject, through the interplay of content and concepts of matter, and familiarization and handling of the main bibliographical sources in the field of genetic engineering and biotechnology to enable the student track and deepening the information received in the classroom.
In the virtual course of the subject there will be available questionnaires of different thematic blocks of the contents of the expository classes for the self-evaluation of the students. These questionnaires will be voluntary and not evaluable for the final grade.
b) The laboratory practices will consist of two compulsory sessions (except for the repeaters) (2h30 ' each) in the laboratory. The practices are of obligatory attendance to 100% (a list of signatures will be taken). Practices consist in performing some of the basic techniques in genetic engineering and will serve to reinforce and see the actual implementation of the contents displayed on the theory program. Students must sign a listing of the group. Learners must make a laboratory practice report with the presentation and discussion of results and respond to a theoretical /practical questionnaire (present in the practical script available on the Virtual Campus) related to conducted/studied laboratory practice (both mandatory and performed by the couple working in the laboratory). This questionnaire is designed to enhance deep learning of the matter, the interrelation of concepts, learn how to transfer and apply knowledge and practice critical thinking and scientific methodology. Must be delivered, in the same document that the report of practices, for evaluation and will be corrected in a tutorial. Estimated non-contact time of practice report and questionnaire preparation is 7,5 hours.
c) The classroom seminars 1, 2 and 3 will include mandatory presentations of students, in periods of about 10' by working group (5 people maximum), from different parts of the seminar concerning the specific topic previously agreed with Professor and supervised by it in the tutorials. In the Virtual Campus, the specific themes and basic figures for carrying out each work will be available for each work group. Each group, in every seminar, must give the teacher a summary of his presentation. This summary, together with the oral presentation itself, and resolution of questions that arise, will be assessed for the final grade. Teacher will choose at the time, in each case, which member or members of the group will present its section. Posted content on the seminars will be added to the theory program contents of the lectures for the exam.
The reports from seminars 1 to 3 once corrected by the teacher will be uploaded, after 1 or 2 weeks, to the Virtual Campus so that the students can check their mistakes, review the advice for improvement, see their evolution and that serve as feedback and self-evaluation.
The classroom seminar 4 consist in the resolution, by the previous working groups, of problems proposed by the teacher. Books and any other teaching and computer material may be used for their resolution, at the teacher's discretion. The questionnaires solved after the session will be delivered to the teacher for evaluation. It does not require more non-face-to-face work than carrying the study of the subject up to date.
All seminars are mandatory attendance (signature sheet) and completion (delivery of reports and bulletin of problems), with a minimum of 80% and availables. Estimated non-attendance time of development of reports for exposure (3 per group work throughout the course) is 15.5 hours.
d) The program of small group tutoring is shown in the Contents section of this guide and these are not mandatory or evaluable. The first tutorial will be virtual (MS-Teams platform) at the direction of the Faculty, at the beginning of the semester following a calendar agreed with the students. The rest of the tutorials, at the request of the students, may be in person or by telematic mechanisms (synchronous on MS-Teams, asynchronous on Virtual Campus and institutional email).
e) The exam is a compulsory face-to-face test for the evaluation of knowledge and skills using a written test in which multiple choice questions, problems similar to those of seminar 4 and development questions will be formulated, covering the topics covered in the expository classes. and in seminars.
Qualification of the student is a weighted average of the performance in the parts where their work is evaluated: exam, performing the reports of seminars, report -questionnaire of practices and active participation in class. The same tools will be used to assess competencies according to the following scheme:
- With the examination the following skills are assessed: CE1.c, CE2.e, CE2.f, CE7.d, CE7.g, CE7.h, CB1, CB2, CB4, CB5, CG1, CG3, CG4, CG5, CT1, CT2, CT4, CT5, CT9, CT10 and CT11.
- In the completion and presentation of works: CE1.c, CE2.e, CE2.f, CE7.d, CE7.g, CB1, CB3, CB4, CB5, CG1, CG4, CG5, CT1, CT2, CT3, CT4, CT5, CT6, CT7, CT8, CT9 and CT10.
- In the laboratory practices report-questionnaire: CE1.c, CE2.e, CE2.f, CE7.d, CE7.g, CB1, CB2, CB4, CB5, CG1, CG2, CG3, CG4, CG5, CT1, CT2, CT3, CT5, CT8, CT9 and CT11.
- With the active participation in the classroom: CE1.c, CE2.e, CE2.f, CE7.d, CE7.g, CE7.h, CB1, CB2, CB3, CB4, CB5, CG4, CG5, CT1, CT2, CT4, CT6, CT7, CT9 and CT11.
Common standards in the evaluation in both calls.
THIS SUBJECT WILL ONLY HAVE EVALUATION OF REPEATING STUDENTS.
The continuous evaluation will suppose 45% of the final grade, distributed in 25% the reports carried out for the seminars, 15% of the memory-questionnaire of practices and 5% for the expositions and answers (seminars 1 to 3) and the active participation and answers bulletin (seminar questionnaire 4). All these evaluable activities are mandatory.
There will be a compulsory face-to-face final exam that represents 55% of the final grade. The subject evaluated in the exam includes the contents of the expository classes and the seminars.
In the second evaluation call, only the compulsory face-to-face final exam will be repeated (which will also represent 55% of the final grade), keeping the grade obtained in the continuous evaluation.
The final exam is considered as a compulsory complementary test to continuous assessment for all students. It will be necessary to achieve a minimum of 4 out of 10 in the final exam (in the two evaluation opportunities) for the continuous assessment to be added. Otherwise, the final grade will be exclusively that of the exam. A minimum final grade of 5 out of 10 will be necessary to pass the course.
There will be no compensatory mechanisms for the non-attendance and / or performance of some mandatory and/or evaluable activity.
Those learners not meeting the required attendance requirements and/or not engage all mandatory activities are not entitled to examination, and therefore cannot pass the subject in either assessment opportunities progress.
The 100% assistance activities per se will not score in the final grade.
Repeating students: repeating students may keep the mark of previous courses of the different sections of the continuous assessment as long as a minimum of 5 out of 10 in each part has been reached, so that they will not be obliged to repeat these activities either and only they will have to do the compulsory final exam (expository classes and seminars). The weighting of each section for the final grade will be the same as that indicated above.
In the case that the above requirements are not met or if the student considers it more appropriate, both a new practice report-questionnaire and new work corresponding to seminars 1 to 3 can be delivered (prior communication with the teaching staff). In both cases, your assessment for the final grade will be the same as the one indicated above. In this situation, the mandatory final exam will account for 60% of the final grade, since it also includes 5% of the mark corresponding to active participation in the classroom (which obviously cannot be done as there is no teaching).
Plagiarism and improper use of technologies in the performance of tasks or tests: in cases of fraudulent performance of exercises or tests, the provisions of the Regulations for the evaluation of student academic performance and review of grades will apply.
The distribution by different means (for example, on web pages, distribution to people outside the course, etc.) without the consent of the teaching staff, of any teaching material available in the virtual classroom is also strictly prohibited.
SUBJECT WITHOUT TEACHING
The most important recommendation is that attendance and use of classes is essential for this system. Reaching the final exam without knowing the teacher and to pass the subject is impossible given the compulsory attendance in some activities. Moreover, even with a 10 on the exam is impossible to pass the course and that weights 55% and there are various classroom and non-contact activities. Bring up the subject, practice thinking (correlating concepts) and deepen the knowledge shown in class is essential to pass the subject.
In the written tests is important to read the questions, interpret the logic of the results or answers, to use precise and rigorous language, and review answers.
Quality and breadth of literature reviewed (if applicable), structure of the answers and presentation (spelling, images, diagrams themselves, etc.), adequacy of the required contents, clarity of exposition and mastery of language and scientific concepts will be assessed in seminar reports and laboratory practices report-theoretical/practical questionnaire.
Likewise, it is recommended to review the test made throughout the course, after evaluation of them (it allows verification and correct errors) and consult with the teacher in tutorials any doubts that may arise.
Make use of the tutorials and contact the teaching staff to solve any doubts and problems that arise in the study of the subject and to monitor the learning.
Communication with teachers
There will be an active virtual classroom of the subject through the Virtual Campus of the USC.
Face-to-face communication in the classroom and in the office. Communications may also be established through institutional email, via the MS-Teams platform, messages through the Virtual Campus forum and by telephone.
Plagiarism and improper use of technologies in the performance of tasks or tests: in cases of fraudulent performance of exercises or tests, the provisions of the Regulations for the evaluation of student academic performance and review of grades will apply.
The distribution by different means (for example, on web pages, distribution to people outside the course, etc.) without the consent of the teaching staff, of any teaching material available in the virtual classroom is also strictly prohibited.
Miguel González Blanco
- Department
- Biochemistry and Molecular Biology
- Area
- Biochemistry and Molecular Biology
- Phone
- 881815386
- miguel.gonzalez.blanco [at] usc.es
- Category
- Professor: University Lecturer