ECTS credits ECTS credits: 6
ECTS Hours Rules/Memories Hours of tutorials: 3 Expository Class: 22 Interactive Classroom: 26 Total: 51
Use languages Spanish, Galician
Type: Ordinary Degree Subject RD 1393/2007 - 822/2021
Departments: Analytical Chemistry, Nutrition and Bromatology
Areas: Analytical Chemistry
Center Faculty of Biology
Call: Second Semester
Teaching: With teaching
Enrolment: Enrollable | 1st year (Yes)
1. To know and know how to apply the basic analytical and instrumental techniques useful in a Biotechnology laboratory.
2. Know how to describe the fundamentals and uses of spectrophotometry, microscopy, mass spectrometry, chromatography and electrophoresis.
3. Know how to identify and interpret spectra of various types for the structural identification of biomolecules and other applications
4. Know how to choose the right technique to solve each particular problem based on analytical criteria
5. Distinguish between chromatographic and electrophoretic separation techniques and select them appropriately for different bioseparation applications.
(Each topic will last for about 2 hours, except for topics 6 and 9 which will last for 3 hours).
Topic 1. Introduction to the basic techniques in Biotechnology
Topic 2. Introduction to spectroscopic methods and classification.
Topic 3. UV-Vis spectrophotometry
Topic 4. Fluorescence spectrophotometry and related techniques
Topic 5. Fundamentals of Nuclear Magnetic Resonance (NMR)
Topic 6. Mass spectrometry (MS)
Topic 7. Introduction to chromatographic separation techniques
Topic 8. Gas chromatography
Topic 9. Liquid chromatographies
Topic 10. Electrophoretic techniques
LAB CLASSES PROGRAM
Lab 1: Basic operations in the analytical laboratory
Lab 2. Applications of UV-Vis Spectrophotometry to Biotechnology
Lab 3: Chromatography Applications to Biotechnology
Lab 4: Applications of Mass Spectrometry and other techniques (NMR&X-ray difraction)to Biotechnology
SEMINARS
Six seminars (11 hours) will be held in small groups, in which exercises, problems and/or simulations of cases related to the contents of the subject will be proposed and solved. Active student participation is required.
TUTORIALS
The tutorials (individual or in groups) will be used to clarify doubts, provide information or guidance to students, as well as to learn about their progress in the acquisition of skills.
BASIC
---SUSAN R. MIKKELSEN. QUIMICA BIOANALITICA: METODOS Y TEORIA ANALITICA PARA EL LABORATORIO DE BIOLOGIA MOLECULAR, FARMACIA Y BIOQUIMICA, 2011 Editorial: UNIVERSIDAD DE BUENOS AIRES. ISBN: 9789502317694
---D. C. HARRIS. "Análisis Químico Cuantitativo". Editorial Reverté, 3ª ed. (6ª del inglés), Barcelona (2007), recomendado cap. 26 págs. 654-666.
---D.A. SKOOG; D.M. WEST; F.J. HOLLER; S.R. CROUCH. “Química Analítica”, Thomson .8ª Ed. Madrid. 2005, cap. 33 págs. 1016-1024.
In addition, throughout the course it will be recommended to visit specific websites with videos and materials of interest for a better understanding of the topics.
COMPLEMENTARY
• “Esenciais USC” Collection
----TÉCNICAS ANALÍTICAS E INSTRUMENTAIS: introdución ás técnicas espectroscópicas: espectro(foto)metría de absorción molecular UV/Vis.
Autoras: Marta Lores Aguín, Carmen García Jares (2019)
---TÉCNICAS ANALÍTICAS E INSTRUMENTAIS: introdución ás técnicas de separación cromatográficas. Autoras: Carmen García Jares, Marta Lores Aguín (2019)
Knowledge/content
Con01: To know the most important concepts, methods and results of the different branches of Biotechnology.
Con04: To know the techniques to determine the properties of proteins and to be able to analyze and manipulate their structure according to their application in Biotechnology.
Abilities/skills
H/D01: Think in an integrated way and approach problems from different perspectives with critical reasoning.
H/D02: Search, process, analyze/interpret and synthesize relevant information and results from various sources and draw conclusions in Biotechnology related topics.
H/D03: Organize and plan their work.
H/D04: Interpret experimental results and identify consistent and inconsistent elements.
H/D05: Work in a team.
H/D06: Maintain an ethical commitment, as well as a commitment to equality and integration.
H/D07: Initiative, entrepreneurial spirit and adaptation to new situations (resilience).
H/D10: Know how to analyze data and interpret experimental results specific to the fields of Biotechnology.
H/D12: To know how to apply instrumental techniques and work protocols in a laboratory, applying the regulations and techniques related to safety and hygiene, waste management and quality.
H/D13: To know how to obtain and interpret information from the main databases (biological, omic, bibliographic) and to use bioinformatics tools.
Competencies
Comp01: That students have the ability to gather and interpret relevant data (usually within their area of study) to make judgments that include reflection on relevant social, scientific or ethical issues.
Comp02: That students are able, both in writing and orally, to discuss and convey information, ideas, problems and solutions related to Biotechnology to both specialized and non-specialized/general audiences.
Comp03: That students have developed those learning skills necessary to undertake further studies with a high degree of autonomy.
Comp04: That students know how to apply the theoretical-practical knowledge acquired in the degree in a professional manner and are competent in the approach/solution of problems, as well as in the elaboration/defense of arguments in both academic and professional contexts related to innovation and the biotechnology industry.
Comp05: To study and learn autonomously, with organization of time and resources, new knowledge and techniques in Biotechnology and acquire the ability to work in a team.
---Master classes (on-site), in which the teacher will explain the concepts of the subject with the support of audiovisual and computer media; it can have different formats (theory, problems and/or general examples, general guidelines of the subject), promoting the participation of the students.
---Seminars (on-site), in small groups, in which theory applications, exercises, problems, case simulations, etc. are proposed and solved. Active participation of students is required.
---Practical laboratory classes (on-site),, in which the students, following the protocols prepared for this purpose, will handle the appropriate equipment and will solve practical questions.
---Tutorials (individual or group, on-site) to clarify doubts, provide information or guidance to students, as well as to learn about progress in the acquisition of skills.
Attendance at seminars, tutorials and laboratory practicals is required.
The evaluation will consist of two parts:
Continuous evaluation:
The continuous evaluation will be done through oral and/or written tests, work delivered and/or presented, student participation in the classroom and in the laboratory. The evaluation of each of the activities to be carried out by the student, to award the final grade, as well as the typology, methods and characteristics of the evaluation system of the subject, are indicated below:
1. Attendance to the interactive classes in small groups (seminars), tutorials and laboratory practices is compulsory.
2. Failure to attend a minimum of 80% of the common hours of seminars and tutorials implies the waiver of the right to continuous evaluation.
3. The attendance to all the laboratory practices will be obligatory, as well as to pass the evaluation. Failure to attend them will prevent the passing of the subject both in the common opportunity and in the opportunity of recovery. In order to pass the subject, the student must obtain a passing grade in the laboratory practices.
Examen final (EF):
The final exam covers all the contents of the subject.
The different activities will count towards the student's final grade as follows:
Final exam 60%.
Continuous evaluation (40%): Seminars 15%; Tutorials 5% and Practicals 20%.
For the evaluation of the laboratory practices, the elements to be evaluated will be the following: Attitude and aptitude in the laboratory, execution of the practical (including presentation of results) and final report.
The continuous evaluation is complementary to the final exam. The student's grade will be obtained by weighting the final exam grade with the continuous evaluation grade; although in order to have the option of weighting it is necessary to have a minimum grade of 4 out of 10 points in the final exam.
All the activities that are qualified as obligatory will be evaluable.
Repeating students who have passed the laboratory practicals will keep the grade obtained for a maximum of two academic years. Therefore, they will not have to take the laboratory practicals again, but they will attend the remaining interactive classes (seminars and tutorials) under the same conditions as the other students.
Evaluation of Competencies: Sem (S) Lab (P) Titorials (T) Final Ex (EF)
Con01: S, P, T, EF
Con04: S, P, T, EF
H/D01: S, P, T, EF
H/D02: S, P, T, EF
H/D03: S, P, T, EF
H/D04: S, P, T, EF
H/D05: S, P, T
H/D06: S, P, T, EF
H/D07: S, P, T
H/D010: S, P, T, EF
H/D012: P
H/D013: S, P
Comp01: S, P
Comp02: S, P, T
Comp03: S, P, T, EF
Comp04: S, P, T, EF
Comp05: S, P, T, EF
Repeating students who have passed the laboratory practicals will keep the grade obtained for a maximum of two academic years. Therefore, they will not have to take the laboratory practicals again, but they will attend the remaining interactive classes (seminars and tutorials) under the same conditions as the other students.
Those repeating students who, having obtained a passing grade in the internship, wish to repeat all or part of it, shall inform the coordinating teacher at the beginning of the course.
FACE-TO-FACE WORK IN THE CLASSROOM HOURS
Large Group Expositive Classes 22
Interactive small group classes (Seminars) 11
Interactive lessons with computer (small group) 00
Tutorials (very small group) 03
Laboratory practices 15
Total hours of classroom or laboratory work 51
STUDENT'S PERSONAL WORK HOURS
Independent individual or group study 44
Resolution of exercises or other work 22
Preparation of laboratory work and preparation of the lab report 30
Total hours of personal work of the student 96
It is advisable to attend the lectures and to use the recommended bibliography, and to carry out the exercises and work proposed.
With regard to the practical part of the course, it is recommended that you read the practice scripts before entering the laboratory.
Registered students need to know and obey the "normas xerais de seguridade nos laboratorios de prácticas" of the University of Santiago de Compostela, to participate in laboratory sessions. This regulation is in the webpage (www.usc.es/estaticos/servizos/sprl/normalumlab.pdf)
A virtual classroom on the Moodle platform (Virtual Campus of the USC, https://cv.usc.es/) will be available to students, which will serve as a repository of the material necessary for the correct development of the subject as well as a channel of communication with students. In addition, another direct communication channel may be operational through the MS-Teams application.
Carmen Maria Garcia Jares
Coordinador/a- Department
- Analytical Chemistry, Nutrition and Bromatology
- Area
- Analytical Chemistry
- Phone
- 881814394
- carmen.garcia.jares [at] usc.es
- Category
- Professor: University Professor
Juan Jose Lopez Mayan
- Department
- Analytical Chemistry, Nutrition and Bromatology
- Area
- Analytical Chemistry
- Phone
- 881814271
- juanjoselopez.mayan [at] usc.es
- Category
- Professor: Temporary supply professor for Special Services and others
Aly Jesús Castillo Zamora
- Department
- Analytical Chemistry, Nutrition and Bromatology
- Area
- Analytical Chemistry
- alyjesus.castillo.zamora [at] usc.es
- Category
- Predoutoral_Doutoramento Industrial
| Tuesday | |||
|---|---|---|---|
| 09:00-10:00 | Grupo /CLE_01 | Spanish | Classroom 04: James Watson and Francis Crick |
| 05.27.2025 16:00-20:00 | Grupo /CLE_01 | Classroom 04: James Watson and Francis Crick |
| 07.01.2025 16:00-20:00 | Grupo /CLE_01 | Classroom 03. Carl Linnaeus |