ECTS credits ECTS credits: 4.5
ECTS Hours Rules/Memories Student's work ECTS: 74.2 Hours of tutorials: 2.25 Expository Class: 18 Interactive Classroom: 18 Total: 112.45
Use languages Spanish, Galician
Type: Ordinary Degree Subject RD 1393/2007 - 822/2021
Center Faculty of Biology
Call: Second Semester
Teaching: Sin Docencia (En Extinción)
Enrolment: No Matriculable (Sólo Alumnado Repetidor)
- Know the general concepts of biotechnological techniques and relating them to other basic and specific subjects of the Degree in Biology.
- Understand the physiological basis and methodology of plant propagation by culture in vitro, and its practical applications in the industry.
- Gain knowledge on the genetic modification of plants, methods, applications and evaluation of them.
- Acquire basic knowledge on the field, both theorical and practical, for its application in later stages of the professional career of the students.
THEORICAL LESSONS: MASTER CLASSES (26 HOURS)
Block 1. Introduction to biotecnology
Unit 1 (2h). Growth and cellular differentiation. Concept of totypotency. Direct and indirect organogenesis. Somaclonal variation and culture instability.
Unit 2 (3h). Introduction to in vitro culture. Establishment of aseptic cultures. Growth media and culture conditions. Plant material: selection and explant asepsis.
Unit 3 (2h). Micropropagation: multiplication, rooting and acclimation. Apical meristem culture. Generation of virus-free plants.
Block 2. Genetics and plant genetic modification. Practical cases and applications
Unit 4 (2h). Transformation mediated by biological vectors: Agrobacterium tumefaciens, A. rhizogenes and viruses.
Unit 5 (1 h). Vectors for plant transformation. Selectable marker genes and reporter genes.
Unit 6 (1h). Transferencia directa de genes. Biolística. Electroporación.
Unit 7 (2 h). Genomics of cultivated species: polyploidy and domestication.
Unit 8 (1 h). Photosynthesis optimization.
Unit 9 (2 h). Nutritional improvement of plants. Biofortification.
Unit 10 (2 h). Fruit maturation.
Unit 11 (2 h). Plants as biofactories.
Unit 12 (2 h). Abiotic resistance.
Unit 13 (3 h). Biotic resistance.
Unit 14 (1 h). Biosecurity. MGOs risks. Legislation.
COMPULSORY LABORATORY PRACTICAL LESSONS (12 HOURS)
Práctica 1 (3 h). Culture media preparation. Media sterilization and additional materials.
Práctica 2 (2 h). Asepsis and starting plant material preparation. Establishment in in vitro culture of aseptic starting cultures.
Práctica 3 (2 h). Working in aseptic conditions (laminar flow bench)
Práctica 4 (3 h). Data collection and transfer of plants to ex vitro conditions.
Práctica 5 (2 h). Data collection and results presentation.
SMALL GROUP TUTORING (3 HOURS)
In the tutoring sessions the students will be assisted on the elaboration of the practical report.
EXAMEN FINAL (2 HOURS)
The final exam will consist on short questions about the theorical and practical contents.
BASIC BIBLIOGRAPHY
Altman, A. and Hasegawa, P.M. (Eds) 2012 Plant Biotechnology and agriculture. Prospects for the 21st century. Elsevier.
Peña, L. (Ed) 2005. Transgenic Plants: Methods and Protocols. Totowa: Humana Press.
Slater, A., Scott, N.W. and Fowler, M.R., 2008. Plant Biotechnology: The Genetic Manipulation of Plants. 2nd ed. Oxford: Oxford University Press.
Smith, R.H., 2012. Plant Tissue Culture: Techniques and Experiments. 3rd ed. San Diego: Academic Press.
COMPLEMENTARY
Al-Khayri, J.M., Jain M.S. and Johson D.V. (Eds) 2016 Advances in Plant Breeding Strategies: Agronomic, Abiotic and Biotic Stress Traits Switzerland. Springer.
Collin, H.A. and Edwards, S., 1998. Plant Cell Culture. Oxford: BIOS Scientific Publishers.
Dixon, R.A. and Gonzales, R.A., 1994. Plant Cell Culture: a Practical Approach. 2nd ed. Oxford: IRL Press at Oxford University.
Dodds, J.H. and Roberts, L.W., 1995. Experiments in plant tissue culture. 3rd ed. Cambridge: Cambridge University Press.
Gresshoff, P.M. (Ed) 1997. Technology transfer of Plant Biotechnology. Boca Ratón, FL: CRC Press.
ON-LINE RESOURCES
Srivastava, P.S, Narula, A. and Srivastava, S. (Eds) 2004. Plant Biotechnology and Molecular Markers. Boston: Springer. Full text available via EBSCOhost Ebooks
In the virtual classroom will include teaching material prepared by the teacher and links to online resources.
Basic competences
All those that are specified in the Report of the Degree in Biology will be developed.
Specific competences
CE1.c - Competence Scope: instrumental knowledge, skills and abilities. Basic biology techniques. Basics of biotechnology in plants
CE7.d - Competence Scope: Biotechnology and biomedicine: Biotechnology and recombinant DNA: Genetic engineering in plants, transgenic plants and foods.
CE7.f - Competence Scope: Biotechnology and biomedicine; Biomarkers, bio tests and crops. Basic techniques used for plant biotechnology.
CE7.h - Competence Scope: Biotechnology and biomedicine: Agrifood and forestry applications.
Transversal competences
CT1 - Capacity for analysis and synthesis.
CT2 - Capacity for reasoning and argumentation
CT3 - Capacity to work in groups and deal with problems in a collective way
CT4 - Capacity to use adequate, diverse and updated information
CT5 - Ability to develop and present an organized and understandable text
CT6 - Ability to give a speech in public in a clear, concise and coherent way
CT7 - Compromise of veracity for the information given to the scientific community
CT8 - Ability in the management of Information and Communications technologies (ICTs)
CT9 - Use of bibliographic resources and Internet information
CT10 - Use of information provided in a foreign language
CT11 - Ability to solve problems by means of the implementation of an integrated knowledge management.
In support of teaching, a virtual classroom will be available on the Moodle platform of the Virtual Campus, to which teaching material will be uploaded and which will be used as a means of communication with students. The monitoring of the continuous evaluation will be carried out through the delivery of works and the completion of questionnaires using the Moodle platform.
Lectures: synchronously in the classroom
Interactive laboratory classes: all the practices will be carried out synchronously in the laboratory. The follow-up of the practices will be carried out through assistance and the preparation and delivery of reports, as well as a final work. These activities are mandatory and evaluable.
Tutorials: They will be face-to-face at previously established times. They may be partially conducted virtually (via Teams) by appointment or at previously established times.
The master classes are not compulsory. The practical lessons are compulsory and part of the final mark. 70% of the grades will be on the exam, 20% on the practical report and 10% on continuous evaluation, which will only be added if 4/10 is reached in the final exam.
The final grade will consist on:
a) Final exam (theory and practice). 70%
b) Continuous evaluation. 30%, divided into the individual practical report (20%) and participation and attitude (10%)
Students from previous years will keep their practical report mark and the continuous evaluation obtained in the last two years, although they can take the practical lessons voluntarily to get access to the continuous evaluation of the present year. The grade of the continuous evaluation of the present year will be kept in the next two academic years as well as in the second opportunity of the final exam during the present year.
Assessment of competences
Final exam: CT1, CT2, CT5, CE1.c, CE7.d, h, f
Practice report (Individual work): CG2 CT1, CT2, CT5, CT7, CT9, CT10, CT11, CE1.c, CE7.f
Collective work: CB3, CG2, CT2, CT3, CT4, CT6, CT7, CT8, CT9, CT10
WORK IN CLASSROOM (HOURS)
Lectures..........................................26
Laboratory Practice / Fieldwork...........12
Small group tutoring...........................3
Exam ...............................................2
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CLASSROOM WORK...........................43
INDIVIDUAL AND COLLECTIVE WORK (HOURS)
Individual study ...............................60
Development of practice report ..........9.5
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PERSONAL WORK.............................69.5
• Attend all teaching activities with active participation in them.
• Consult the recommended bibliography.
• Delve into the issues.
• Make frequent use of tutorials for any issue related to the subject.
Te subject will count with a virtual classrom in the Moodle platform. The communication with the students will be through email, the Moodel platform and/or MS-teams.
For cases of fraudulent performance of exercises or tests, the provisions of the Regulations for the assessment of academic performance of the students and the review of qualifications will apply.
| 06.05.2025 10:00-14:00 | Grupo de examen | Classroom 12.Vasili Dokucháyev |
| 07.09.2025 10:00-14:00 | Grupo de examen | Classroom 12.Vasili Dokucháyev |