Genetic Engineering

- To identify the methods and applications of recombinant DNA technology.
- To identify the most used techniques and strategies for the production of recombinant proteins.
- To number gene-editing and genome sequencing technologies.
- To identify and apply experimental protocols for the management and analysis of nucleic acids.
-To know how to clone and express recombinant proteins.

Lectures(27 h)

BLOCK I. ESSENTIALS TOOLS AND BASIC TECHNIQUES (9 h)
Unit 1. Introduction to genetic engineering (1 h)
Unit 2. Enzymology of recombinant DNA (3 h)
Unit 3. Basic techniques for isolation, purification and analysis of nucleic acids (2.5 h)
Unit 4. PCR (2.5 h)

BLOCK II. MOLECULAR CLONING (9 h)
Unit 5. Molecular cloning in bacterial cells (4.5 h)
Unit 6. Libraries (2 h)
Unit 7. Molecular cloning in eukaryotic cells (2.5 h)

BLOCK III. DNA MODIFICATION AND EXPRESSION OF RECOMBINANT DNA (9 h)
Unit 8. Expression of recombinant DNA (4 h)
Unit 9. Site-directed mutagenesis. Introduction to protein engineering (2 h)
Unit 10. Gene inactivation and gene editing. Transgenic organisms. (3 h)

Seminars (9 h)
-Seminars 1, 2: problems and questions concerning units 2 and 3. (2 h)
-Seminar 3: management of databases and computer programs. (1 h)
-Seminar 4 and 5: problems and exercises related to DNA cloning and expression (4 h)
-Seminar 6: presentation of a work that will cover topics explained throughout the course. (2 h)

Each student will have four work sessions in which they will perform:
-Session 1: Cloning of a DNA fragment in a vector. Transformation of E. coli cells and selection of transformants.
-Session 2: Plasmid purification, digestion with restriction enzymes and analysis in agarose electrophoresis.
-Session 3. Directed mutagenesis. Yeast transformation.-
Session 4: Analysis and interpretation of the results.
-Exam (3 h)

Basic bibliography
-Brown, T.A., 2016. Gene cloning and DNA analysis: An Introduction. 7th ed. Chichester: Wiley-Blackwell.
-Clark, D.P. and Pazdernik, N.J., 2016. Biotechnology. [on-line] 2nd ed. Amsterdam: Elsevier Academic Press. Available at: https://www-sciencedirect-com.ezbusc.usc.gal/book/9780123850157/biotech…
-Perera, J., Tormo, A. y García, J.L., 2002. Ingeniería genética. 2 v. Madrid: Síntesis.
-Real García, M.D., Rausell Segarra, C. y Latorre Castillo, A., 2017. Técnicas de ingeniería genética. Madrid: Síntesis.

Complementary bibliography

-Clark, D.P, 2019. Molecular Biology. [on-line] 3rd ed. Amsterdam: Elsevier. Available at: https://www-sciencedirect-com.ezbusc.usc.gal/book/9780128132883/molecul…
-Glick, B.R. and Patten, C.L., 2017. Molecular biotechnology: principles and applications of recombinant DNA. 5th ed. Washington DC: ASM Press.
-Green, M.R. and Sambrook, J., 2012. Molecular cloning: a laboratory manual. 4th ed. 3 v. Cold Spring Harbor: Cold Spring Harbor Laboratory Press.
-McPherson, M.J. and Møller, S., 2006. PCR. 2nd ed. New York: Taylor & Francis.
-Renneberg, R., Berkling, V., and Loroch, V., 2016. Biotechnology for beginners [on-line]. Amsterdam: Elsevier. Available at: https://www-sciencedirect-com.ezbusc.usc.gal/book/9780128012246/biotech…

Other resources
- http://biomodel.uah.es. Web site of the Universidad Alcalá de Henares
- http://www.ncbi.nlm.nih.gov/guide/. The National Center for Biotechnology Information, (NCBI) main source of data Banks (nucleotides, genes and genomes) and bioinformatics applications.
-Electronic resources from BUSC: Cell, Nature, Science, Sciencedirect, etc.

Knowledgments/Contents: Con01, Con05
Habilities: H/D02, H/D10, H/D12
Competences: Comp02, Comp03, Comp05

-MASTER LECTURES: will be held face-to-face; the teacher, through a master lesson and with the support of slides and course material available on the virtual campus, will explain de fundamental issues of the subject. Non-compulsory activity.
-SEMINARS face-to-face in small groups, where the students, through cooperative learning, will solve problems or issues. The students will also prepare a topic in small groups and also could discuss topics related to the subject. Non-compulsory activity.-Small group TUTORIALS (2 sessions of 1 h): clarification of concepts or doubts about the lectures, seminars or practices. Non-compulsory activity.
-LABORATORY PRACTICAL CLASSES, in which the students, following the protocols prepared for this purpose, will handle the appropriate equipment and solve practical questions. Each student will have four work sessions in the laboratory. Mandatory activity.

1.The evaluation will consist of two parts:

1.1) Continuous evaluation (30% of the final grade), which in turn consists of:

i.Seminars (20% of the final grade). NON-MANDATORY ACTIVITY THAT CAN BE EVALUATED.The evaluation will be based on the exercises / reports presented to the teacher or carried out in the seminars: seminars 1, 2 y 3=2% each, seminars 4 y 5=4% and seminar 6=6%. The lack of attendance at a 25% of the seminar hours implies the waiver of the right to seminars evaluation.

ii.Laboratory practices (10% of the final grade). EVALUABLE MANDATORY ACTIVITY.For the grade, a final report and/or questions will be scored. Unexcused absences will imply a qualification of NOT SUITABLE. Students must obtain a qualification higher than 4 in the practices to overcome the subject.The control of attendance to face-to-face activities will be by signature.

1.2) Final Test (70% of the final grade). EVALUABLE MANDATORY ACTIVITY, COMPLEMENTARY TO THE CONTINUOUS EVALUATION.It will consist of an exam with multiple-choice test and questions / exercises related to the theoretical and practical content of the subject.IThe final qualification of the student will be obtained by weighing the final test with the continuous assessment (it is necessary to have a minimum score of 4.5 out of 10 points in the final exam to do the weighing). Otherwise, the final grade will be exclusively that of the exam (70% of the total grade).

2.Students who fail the course in the ordinary exam opportunity must retake the final test during the recovery opportunity (2nd opportunity). Scoresobtained for the continuous evaluation will be maintained for the recovery opportunity. Those students who suspend the practices must do a recoverytest.

3.Students who do not pass the subject but who have passed the laboratory practices will maintain those marks for the next academic year. Studentswho are enrolled in the class for the second time or more will be evaluated following the same guidelines for attendance to classes and calculating thefinal grade as students enrolled in the class for the first time.

4. Learning outcomes evaluation:
-Exam: Con01, Con05, Comp02, Comp03.
-Seminars: H/D02, H/D10, Comp02, Comp03, Comp05.
-Practical classes: Con05, H/D10, H/D12, Comp05.

In general, an average of 150 hours of work is estimated for this subject, of which 51 hours correspond to attendance at theoretical and practical classes, seminars and tutorials, 96 hours to self-employment of the student and the remaining 3 to the performance of exams and review of them.

- Assistance and participation in the proposed activities, as well as bringing the matter up to date.
-Consult of the recommended bibliography.
-Use of the virtual classroom.
-To make use of the tutorials for any type of matter related to the subject.

For a better understanding of this subject, the student must have knowledge of Biochemistry I and first topics of Biochemistry II, Genetics I and Genetics II (gene expression).
There will be an operational virtual classroom in the Moodle platform with material related to classes, seminars and practices. Announcements will also be made through this platform, so students should check their institutional e-mail frequently.
The preferred channels of communication will be e-mail or individual tutoring. If necessary, communication can be established through MS-Teams.
IT IS EXPRESSLY FORBIDDEN FOR STUDENTS TO DISTRIBUTE TEACHING MATERIAL (BOTH WRITTEN AND AUDIOVISUAL) TO PEOPLE OUTSIDE THE COURSE.