Analytical separation and chromatography techniques

Acquisition of knowledge and skills about chromatographic and other analytical separation techniques, including theoretical aspects and its application to solve real-life problems.

I.-ADVANCES IN GAS CHROMATOGRAPHY
• Advances in instrumentation (injectors, columns, etc.)
• Ultrafast chromatography (fast GC)
• Multidimensional gas chromatography (GC-GC, GCxGC, etc)
• Selected examples

II-ADVANCES IN LIQUID CHROMATOGRAPHY
• Fast liquid chromatography and “ultra-high" resolution liquid chromatography (UHPLC or UPLC)
• New columns and new separation modes (core-shell columns, HILIC, high temperature, etc.).
• Multidimensional liquid chromatography (LC-LC, LCxLC, etc)
• Capillary liquid chromatography and nano-LC
• Applications and selected examples

III-CAPILLARY ELECTROPHORESIS
• Principles of electrophoresis
• Instrumentation in capillary electrophoresis
• Modes of capillary electrophoresis and electromigration techniques
• Selected examples

IV.- FIELD FLOW FRACTIONATION (FFF)
• Introduction to FFF
• Instrumentation
• Types of fields most commonly applied (gravitational, thermal...)
• Operation modes (normal, steric...)
• Selected examples

On-line basic resources:
CHROMACADEMY: www.chromacademy.com

Complementary literature:
I.-ADVANCES IN GAS CHROMATOGRAPHY
• Colin F. Poole (Ed.) Gas Chromatography. Amsterdam. Elsevier Science
Publishing, 2012.
• Colin F. Poole (Ed.). The Essence of chromatography. Amsterdam.
Elsevier, 2003

II-ADVANCES IN LIQUID CHROMATOGRAPHY
• Q. Alan Xu. Ultra-High Performance Liquid Chromatography and its applications. New York.
Wiley. 2013
• Colin F. Poole (Ed.). The Essence of chromatography. Amsterdam.
Elsevier, 2003

III-CAPILLARY ELECTROPHORESIS
• P. Schmitt-Kopplin (Ed.). Capillary electrophoresis: methods and
protocols. Totowa, NJ : Humana Press, 2008
• James P. Landers (Ed.). Handbook of capillary electrophoresis. Boca
Raton. CRC Press, 1997.
• C. Cruces Blanco. Electroforesis Capilar. Almería. Servicio de
Publicaciones de la Universidad de Almería. 1998.
• Colin F. Poole (Ed.). The Essence of chromatography. Amsterdam.
Elsevier, 2003

IV.- FIELD FLOW FRACTIONATION (FFF)
• Martin E. Schimpf, Karin Cadwell, J. Calvin Giddings (Eds). Field-flow
fractionation handbook. New York. John Willey & Sons, 2000.
• Josef Janca. Field-flow fractionation: analysis of macromolecules and
particles. New York. Marcel Dekker, 1988.

General skills
CG2 – To identify information from scientific literature using the appropriate channels and integrate this information to raise and contextualize a topic of research
CG4 - To demonstrate ability to analyze, describe, organize, plan and manage projects
CG5 - To use scientific terminology in English language to discuss the experimental results in the context of the chemical profession
CG7 - To be able to work in a team and adapt to multidisciplinary groups
CB7 - That students know how to apply the acquired knowledge to problem solving in new or little known environments within broad (or multidisciplinary) contexts related to their field of study
CB9 - That students know how to communicate their findings and reasoning to specialized and non-specialized public in a clear and unambiguous way
CB10 - That the students have the learning skills allowing them to continuing studying in an autonomous way
Transferable skills
CT1 Elaborate, write and publicly defend scientific and technical reports.
CT3 Work with autonomy and efficiency in the daily practice of research or professional activity.
CT4 Appreciate the value of quality and continuous improvement, acting with rigor, responsibility and professional ethics.

Specific skills
CE2 - To propose alternatives for the resolution of chemical complex problems from different areas of chemistry
CE4 - To innovate in chemical analysis methods related to the different areas of chemistry
CE6 -Design processes involving the treatment or disposal of hazardous chemicals
CE7 - To operate with advanced analytical instrumentation
CE9 - To value, promote and practice innovation and entrepreneurship in industry and in chemical research.

Master lectures complemented with virtual teaching tools (USC virtual campus)
Seminars from master lecturing staff and invited professionals.
Problem solving and case study seminars
Individualized and small group tutorial classes.
Presentation of short case-study assignments.
Self-based study.
Short tests.

The evaluation of this matter will be performed by means of continuous assessment and a final examination. Access to these examination is limited by attending at least to 80 % of educational activities at. The continuous assessment (N1) will have a 45% weight of the total assessment and will consist of two components: interactive classes in small group (seminar lectures) and interactive classes in very small group (tutorial lectures).

The seminar and the tutorial lectures will include the following elements:
• Problem and practical cases-solving: 15 %
• Accomplishment of works and written reports: 10 %
• Oral presentations: 10 %
• Continuous assessment of questions response during the course: 10 %

The final examination will cover the totality of the contents of the course
The qualification of the student will be obtained by applying the following formula:
Final qualification = 0.45xN1 + 0.55xN2
Where:
N1= continuous evaluation (0-10 scale)
N2= final examination marks (0-10 scale)
Assesment of Skills: Problem Solving and Practical Cases-solving (R-PCP), Assignments and Reports (TI), Oral presentations (EO), Final Exam (EF), Continuous Assessment, response to questions during the course (PCO)
CG2: TI, PCO
CG4: TI, PCO
CG5: TI; EO
CG7: TI
CB7: EO
CB9: EO
CB10: EF
CT1: EO, TI
CT3: EO
CT4: EO
CT5: EF
CE2: EF
CE4: EF
CE6: EF, R-PCP
CE7: EF, R-PCP
CE9: EF, R-PCP

CLASSROOM:
Master lectures: 12 h
Interactive small group classes (seminars): 7 h
Very small group tutorials: 2 h
Total hours face-to-face classroom hours: 21 h

SELF-STUDY:
Individual or group self-study: 24 h
Exercise solving and related work: 12 h
Preparation of oral presentations and/or written assignments. Library or similar activities: 18 h
Total hours student's personal work: 54 h

Attendance to all lecturing activities.
To devote time for the personal study.
To consult and discuss any doubt with the lecturing staff.
Active participation during all lecturing activities.