ECTS credits ECTS credits: 3
ECTS Hours Rules/Memories Hours of tutorials: 2 Expository Class: 12 Interactive Classroom: 7 Total: 21
Use languages Spanish, Galician
Type: Ordinary subject Master’s Degree RD 1393/2007 - 822/2021
Departments: Analytical Chemistry, Nutrition and Bromatology
Areas: Analytical Chemistry
Center Faculty of Chemistry
Call: Second Semester
Teaching: With teaching
Enrolment: Enrollable | 1st year (Yes)
Course objectives
Learning Objectives
• Complete acquisition of all aspects of interest on analytical mass spectrometry for qualitative and quantitative purposes.
• This course will provide basic and applied knowledge on modern analytical techniques based on mass spectrometry mainly focused on organic compounds analysis. Also, it will provide students with criteria for (1) selecting the mass spectrometry technique most suitable to solve each analytical problem and for (2) interpreting the results. Relevant issues of the course are explaining the physical and chemical fundamentals of the techniques described, the instrumental configuration, the experimental conditions and some relevant applications
• The student will know the main ionization sources in mass spectrometry, identifying the main features of the information generated by each of these ionization techniques, as well as the basic mechanisms involved in the generation of ions in each case.
• Differentiate the mass analyzer according to their operating principles.
• Understand and interpret the analytical properties that define the characteristics of interest of instrumental methods based on mass spectrometry.
• interpretation of mass spectra of organic compounds.
• Interpretation and explanation of results based on theoretical knowledge acquired, as well as those obtained in the laboratory and from literature search.
• Manage databases spectra for qualitative purposes and understand the philosophy of the different approaches used to obtain quantitative results using mass spectrometry techniques.
• Understand the roll of stable isotopes labelled species in qualitative and quantitative applications of mass spectrometry.
• Understand the interactions between mass spectrometry and continuous separation techniques, in particular chromatographic techniques for the determination of trace compounds in complex samples.
Chapter 1. Introduction to mass spectrometry.
Chapter 2. Ionization sources: operational fundaments and instrumentation
Chapter 3. Mass Analyzers: operational principles, instrumenation and main features.
Chapter 4. Hybridation with chromatography techniques.
Recommended bibliography
Reference book
J. H. Gross, Mass Spectrometry: a textbook, Springer, 3a ed. 2017
Supplementary bibliography
- E. de Hoffmann, V. Stroobant, Mass Spectrometry: Principles and Applications, 3a ed., Wiley, 2007
- A. E. Ashcroft, Ionization Methods in Organic Mass Spectrometry, Royal Society of Chemistry, 1997
- C. Dass, Fundamentals of Contemporary Mass Spectrometry, Wiley, 2007
- S. Shrader, Introductory Mass Spectromety, CRC Press, 2a ed., 2014
- W.M.A. Niessen, R.A.C. Correa, Interpretation of MS-MS Mass Spectra of Drugs and Pesticides, Wiley 2017. On-line available.
- K. Downard, Mass Spectrometry: a Foundation Course, RSC, 2004
- R. M. Smith, K.L. Busch, Understanding Mass Spectra- A basic Approach, John Wiley & Sons, 1999.
- R.K. Boyd, C. Basic, R.A. Behen, Trace Quantitative Analysis by Mass Spectrometry, John Wiley and Sons, 2008.
- B. Ardrey, Liquid Chromatography-Mass spectrometry: an introduction, Wiley, 2003
- R. Willoughby, E. Sheehan, S. Mitrovich, A global view of LC/MS, Global View Publishing, 2nd edition, 2002.
- M. C. McMaster, GC/MS, A Practical User´s Guide, John Wiley & Sons, 2008.
- W.M.A. Niessen, Liquid Chromatography-Mass spectrometry, Taylor and Francis, 2007
Basic and general skills
• To search information in the scientific literature using the appropriate channels and integrate this information to develop and to contextualize a research topic.
• Responsability in the management of information and knowledge in the field of Industrial Chemistry and Chemical Research.
• To demonstrate the ability to analyze, describe, organize, plan and manage projects.
• Use scientific terminology in English to argue experimental results in the context of the chemical profession.
• Correctly apply new information gathering and organization technologies to solve problems in professional activity.
• To assess the human, economic, legal and technical dimension in professional practice, as well as the impact of chemistry on the environment and on the sustainable development of society.
• That students are able to integrate knowledge and face the complexity of formulating judgments based on information that, being incomplete or limited, includes reflections on the social and ethical responsibilities linked to the application of their knowledge and judgments.
• That the students know how to communicate their conclusions and the latest knowledge and reasons that support them to specialized and non-specialized audiences in a clear and unambiguous way.
Specific skills
• Ability to demonstrate knowledge and understanding of the operating principles corresponding to different mass spectrometry modalities aiming organic compounds analysis.
• Ability to apply such knowledge to select the most appropriate analytical technique for each particular problem.
• Ability to apply this knowledge to solve qualitative and quantitative problems following previously developed models.
• The ability to discriminate the possibilities of mass spectrometry as determination techniques in combination chromatographic processes versus conventional detectors.
Basic and general skills
• Ability to manage and to communicate, both in writing and orally, learned topics
• Ability to organize and plan the fundamental concepts of chemistry, so as they allow facing new problems.
• Ability to study and learn independently.
• Skills in finding information.
Specific skills
• Ability to demonstrate knowledge and understanding of the operating principles corresponding to different mass spectrometry modalities aiming organic compounds analysis.
• Ability to apply such knowledge to select the most appropriate analytical technique for each particular target.
• Ability to apply this knowledge to solve qualitative and quantitative problems following previously developed models.
• The ability to discriminate the possibilities of mass spectrometry as determination techniques in combination chromatographic processes versus conventional detectors.
• Skill in assessing and interpreting the generated information and chemical data.
• Ability to handle chemical reagents and organic compounds safely.
• Acquisition and use of information and technical literature relating to the analysis of organic compounds by mass spectrometry.
• Ability to understand the possibilities, and the limitations, of mass spectrometry and hybrid techniques in the analysis of organic compounds in different real-life matrices.
Transversal competences
• Prepare, write and publicly present scientific and technical reports.
• Work autonomously and efficiently in the daily practice of research or professional activity.
• Appreciate the value of quality and continuous improvement, acting with rigor, responsibility and professional ethics.
Theoretical face-to-face classes. Expository lectures (use of blackboard, computer, cannon) complemented with virtual teaching tools.
Seminars held with teachers from the Master, or with invited professionals from the industry, the administration or from other Universities. Interactive sessions related to different subjects with debates and exchange of opinions with students.
Solving practical exercises (problems, test questions, interpretation and processing of information, evaluation of scientific publications, etc.).
Individual or small group tutoring.
Oral presentation of papers, reports, etc., including debate with teachers and students.
Personal study based on different sources of information.
Carrying out different tests to verify the acquisition of both theoretical and practical knowledge and the acquisition of skills and attitudes.
Theoretical face-to-face classes. Expository lectures (use of blackboard, computer, cannon) complemented with virtual teaching tools.
Seminars held with teachers from the Master, or with invited professionals from the industry, the administration or from other Universities. Interactive sessions related to different subjects with debates and exchange of opinions with students.
Solving practical exercises (problems, test questions, interpretation and processing of information, evaluation of scientific publications, etc.).
Individual or small group tutoring.
Oral presentation of papers, reports, etc., including debate with teachers and students.
Personal study based on different sources of information.
Carrying out different tests to verify the acquisition of both theoretical and practical knowledge and the acquisition of skills and attitudes.
Assessment procedure
Assessment of this matter will consist of both, continuous evaluation and a final exam. Only those students attending to at least 80% of obligatory lectures (interactive classes and tutorials) have the right of a final examination. Continous evaluation (N1) contributes to 30% of the final mark and it will consist on two contributions (interactive classes) and tutorials. Interactive classes and tutorials will include problems and practical cases solving (20% contribution) and active participation during interactive and expositive classes will account up to 10% of the continuous evaluation.
The final exam (N1) will cover all contents of the matter.
The final mark of each student will be calculated accordingly to the following mathematical expression:
Final mark= 0.30 x N1 + 0.70 x N2.
Being, N1 the numeric value of the continuous evaluation (scale from 0 to 10) and N2 the numerical mark of the final exam (scale from 0 to 10). Students coursing the matter in second and further academic years are under the same attendance regimen as novel students.
Recommendations to pass the evaluation:
Students are encouraged to regularly check theoretical issues corresponding to the different chapters of the matter and to use the recommended bibliographic sources. The degree of successful when solving the proposed exercises will provide the students valuable clues about their competence in the different topics covered by this matter. Students with relevant difficulties to manage the proposed activities should contact the teacher, during his/her tutorial hours, to analyze their limitations and to try to overcome them.
In case of detecting the fraudulent performance of exercises and/or assessment tests, the Regulations for evaluating the academic performance of students and reviewing grades will be applied.
FACE TO FACE HOURS IN THE CLASSROOM
Lectures in large groups 12 h
Interactive lessons in small groups (seminars) 7 h
Tutorials (very small groups) 2 h
Total hours in the classroom or in the laboratory 21 h
AUTONOMOUS STUDENT ACTIVITIES
Self-study hours or in small groups 48 h
Solving exercises, or other assignments 28 h
Preparation of oral presentations, written
assigments or exercises. Activities in the library or similar 8 h
Total hours of autonomous work 84 h
- It is important to attend to master lectures and interactive activities.
- It is recommended to revise the explained contents daily during course development.
Isaac Rodriguez Pereiro
Coordinador/a- Department
- Analytical Chemistry, Nutrition and Bromatology
- Area
- Analytical Chemistry
- Phone
- 881814387
- isaac.rodriguez [at] usc.es
- Category
- Professor: University Professor
Maria Ramil Criado
- Department
- Analytical Chemistry, Nutrition and Bromatology
- Area
- Analytical Chemistry
- Phone
- 881816035
- maria.ramil [at] usc.es
- Category
- Professor: University Lecturer
| Tuesday | |||
|---|---|---|---|
| 16:00-18:00 | Grupo /CLE_01 | Spanish | Analytical Chemistry Classroom (2nd floor) |
| Thursday | |||
| 16:00-18:00 | Grupo /CLE_01 | Spanish | Analytical Chemistry Classroom (2nd floor) |
| 05.15.2025 10:00-14:00 | Grupo /CLE_01 | Classroom 2.12 |