ECTS credits ECTS credits: 4.5
ECTS Hours Rules/Memories Student's work ECTS: 74.25 Hours of tutorials: 2.25 Expository Class: 18 Interactive Classroom: 18 Total: 112.5
Use languages Spanish, Galician, English
Type: Ordinary Degree Subject RD 1393/2007 - 822/2021
Departments: Analytical Chemistry, Nutrition and Bromatology
Areas: Analytical Chemistry
Center Faculty of Chemistry
Call: Second Semester
Teaching: With teaching
Enrolment: Enrollable
The student should acquire expertise on
1. Analytical techniques and procedures in kinetic and catalytic (enzymatic catalysis included) methods and in immunoassays.
2. Principles of automation and knowledge on basic and advanced instrumentation in automated systems, and on hyphenated techniques in analytical laboratories.
3.-Using theoretical and practical knowledge to plan, apply and manage the most appropriate analytical methodology to face problems related to environmental, sanitary, industrial, and food issues, or other related to chemical substances.
4. Scientific literature and technical information management related to analytical chemical processes.
Theoretical lessons:
Unit 1. Hyphenated techniques
Lesson 1. Hyphenated techniques I
Introduction. Non-chromatographic separation techniques on line spectrometric techniques. Chromatographic separation techniques on line atomic spectrometric techniques: GC/HPLC - AES/AAS/AFS. Chromatographic separation techniques on line molecular spectrometric techniques: GC-FTIR, GC-NMR
Lesson 2.- Hyphenated techniques II
Mass spectrometry based hyphenated techniques: instrumentation. Atomic mass spectrometry based hyphenated techniques: HPLC-ICP-MS, GC-ICP-MS, CE-ICP-MS. Molecular mass Spectrometry based hyphenated techniques: GC-MS, HPLC-MS, HPLC-MS/MS (tandem mass spectrometry)
Unit 2. Kinetic methods of analysis
Lesson 3. Introduction to kinetic methods of analysis
Introduction to kinetic methods of analysis. Suitable chemical reactions in Analytical Chemistry: pseudo-first order reactions. Factors that affect reaction rates. Analytical use of reaction rates: determination of a single species, simultaneous determinations.
Lesson 4. Catalytic kinetic methods
Catalytic kinetic methods. Types of catalysis. Non-enzymatic catalytic reactions. Micellar catalysis. Analytical use of catalytic reactions
Lesson 5. Enzymatic catalytic methods
Introduction to enzymatic catalytic methods. Activators and inhibitors of the enzyme’s activity. Analytical use of the Michaelis-Menten equation: KM and kcat. Factors that affect the enzymatic reaction rate. Instrumentation for kinetic methods of analysis.
Unit 3. Immunoassay techniques
Lesson 6. Immunoassay techniques
Introduction. Basis of the immunoassay techniques. Types of immunoassay techniques. Homogeneous and heterogeneous immunoassay. Types of markers. Enzyme immunoassay (EIA)
Unit 4. Automation
Lesson 7. Introduction to automation in Analytical Chemistry
Definition and objectives of automation. Applicability of automation in Analytical Chemistry. Problems derived from automation. Quality, automation and Analytical Chemistry. Automation of the previous stages of the analytical process.
Lesson 8. Total automation: Analyzers
Analyzers: definition and classification. Continuous analyzers. Discontinuous analyzers. Robotic stations. Process analyzers
Lesson 9. Automation using flow techniques
Types of flow techniques: segmented flow analysis (SFA), flow injection analysis (FIA), sequential injection analysis (SIA). Theoretical basis of flow techniques.
Interactive lessons:
Interactive lesson I. Hyphenated techniques I
Interactive lesson II. Hyphenated techniques II
Interactive lesson III. Kinetic methods of analysis
Interactive lesson IV. Catalytic kinetic methods
Interactive lesson V. Enzymatic catalytic kinetic methods
Interactive lesson VI. Immunoassay techniques
Interactive lesson VII. Immunoassay techniques (simulations in the computer lab)
Interactive lesson VIII. Automation
Lab lessons:
1.- Identification and determination of polycyclic musks in personal care products by GC-FID and GC-MS
2.- Catalytic spectrophotometric determination of iodine in table salt
3.- Automated determination of total iron in iron supplements by FIA-spectrophotometry
Basic literature:
- Analytical Chemistry, R. Keller, J.M. Mermet, M. Valcárcel, M. Otto, H.M. Widmer, Wiley, 2004
-Automatización y miniaturización en Química Analítica, M. Valcárcel, M.S. Cárdenas, Ed Springer,2000
-Química Analítica: métodos cinéticos, inmunoensayo y análisis de trazas, M. Llompart Vizoso, I. Rodriguez Pereiro, L. Sánchez Prado, 2010
Additional literature:
-Principios de Análisis Instrumental, 6ª Ed., Skoog, Holler, Nieman, Ed. Thonsom-Paraninfo, 2009.
-Análisis Químico de Trazas, C. Cámara, C. Pérez-Conde (Eds.), Ed. Síntesis, 2011.
General competences:
CG2.- Graduates will be able to obtain and interpret data, information and relevant results; they could reach conclusions and elaborate reports about scientific, technological or other type of problems requiring knowledge about Chemistry
CG3.- Graduates will be able to apply theoretical and practical knowledge, analytical and abstraction skills to the definition and solution of problems that can be found in the academic and in the professional field.
CG4.- Graduates will acquire the ability to communicate (oral and writing communication) knowledge, procedures, results and ideas in Chemistry to several types of audiences.
CG5.- Graduates will improve an autonomous learning and time management, information, new knowledge and techniques that are used in scientific/technological field
CG1 - Graduates will be able to understand the most important concepts, methods and results of the different disciplines of Chemistry, and they will have a historical perspective of development of this science
Transferable competences:
CT1.- Improve the ability to synthesize and analyze information
CT2.- Develop their organization and planning abilities
CT3.- Acquire knowledge of a foreign language
CT4.- Improve problem solving skills
CT5.- Increase the ability of taking decisions
Specific competences:
CE14.- Solve qualitative and quantitative problems following models previously developed.
CE18.- Follow standard lab protocols to analyze or synthesize organic or inorganic systems.
CE19.- Use some of the basic analytical chemical instrumentation to obtain structural information, separate compounds, etc
CE20.- Interpret experimental data obtained from the measurements performed in the lab and discuss the significance of the results in relation with the concepts studied during the course
CE24.- Understand the qualitative and quantitative aspects of the chemical problems
The teaching of this subject will consist of theoretical lectures which allow the students to work and learn by themselves.
These theoretical lectures will be complemented with seminar classes in which the teacher will solve practical problems and doubts arising from the students’ personal work may pose when solving the exercises or questions proposed by the teacher. In addition, the student must solve exercises proposed by the teacher during the seminar classes that will be used to assess the level of understanding and knowledge of the subject.
The lab lessons will be taught in four hours laboratory sessions for 3 days. The student's knowledge of the lab sessions will be previously assessed before the students start to work in laboratory.
The virtual platform and the virtual campus will be used to leave supporting material, web addresses, etc., and to facilitate communication among teachers and students.
The following teaching methodologies will be used during the lessons:
i) Lectures: Theoretical lessons to explain the basis of each topic, that will be illustrated with examples and related problems. They will be supplemented with the work of the student in the interactive lessons and the self-study learning.
ii) Interactive sessions (Seminars and Group tutorials). The lecturer will give the student sets of problems or case studies for the seminars. The lecturers will solve some of the problems in the board, and the students will be also encouraged to solve, present and discuss similar exercises. Some of the exercises done by the students will be collected for the continuous assessment. Interactive sessions (especially group tutorials) can be also used for supervision, presentation and discussion of the academic works proposed during the term, or to discuss course work or to clear up points arising from the lectures and seminars
Attendance at the interactive lessons is mandatory.
iii) Lab lessons. There will be three lab sessions of 4 hours.
Attendance at the lab sessions is mandatory. The students should read the experiment they are going to perform during each lesson and show their knowledge in the initial test.
The explanations will be supplemented with basic teaching resources and new technologies: use of the virtual platform, videos, slide presentations, ...
iv) Individualized tutoring: they will be done through the MS Teams platform
The assessment will consist of two parts:
i) Continuous assessment: 30 %:
i.1) Exercises and other activities in the class: 15 %
i.2) Lab lessons (organization and execution of the experiments, final report): 15 %
ii) Final exam: 70 % (the exam will be face-to-face).
Continuous assessment will be considered for the final mark when the student has obtained the minimum mark of 5.0 in both i.1) Exercises and other activities in the class, and i.2) Lab lessons (organization and execution of the experiments, final report).
To be able to pass the subject considering continuous evaluation it is essential to obtain a grade higher than 4.0 in the exam.
The final mark obtained will not be lower than that obtained in the final examination.
The student must pass the laboratory part to pass the course. If the students have passed the laboratory part in the two previous years, they need not to attend again to the laboratory sessions.
The following competences will be evaluated in the different activities:
Interactive lessons: CG2, CG3, CG4, CG5, CT1, CT2, CT4, CT5, CE14, CE24
Lab lessons: CG2, CG3, CG4, CT1, CT2, CT4, CT5, CE18, CE19, CE20, CE24
Group tutorials: CG4, CT1, CT2
Exam: CG2, CG3, CG4, CT1, CE14, CE19, CE20, CE24
The final exam will encompasses the 100% of the mark for those students that do not pass the continuous assessment.
Indication referring to plagiarism and the improper use of technologies in carrying out tasks or tests: “For cases of fraudulent execution of exercises or tests, resolutions from the ‘Normativa de avaliación do rendemento académico dos estudantes e de revisión de cualificacións’ will be applied.
Presential learning
40 hours consisting of:
Lectures: 18 h
Interactive lessons in smaller groups (Seminars): 8 h
Interactive lessons in small groups (Group tutorials): 2 h
Lab lessons: 12 h
Self-study learning (72.5 h)
In groups, or individual self-study: 36 h
Solution of exercises and other assignments: 10 h
Prepare presentations, written assignments, exercises, bibliographic searches, etc: 15.5 h
Preparation for lab sessions, answer the questions after the experiments, etc : 11 h
Recommendations for the study of the subject
1) Class attendance is highly recommended
2) Use the recommended literature. Try to solve the set of problems or prepare the activities proposed by the lecturer before the seminars
3) Read the lab manual and prepare the lab lessons in advance
It is recommended to have passed the following subjects: Técnicas Analíticas de Separación (G1041226) y Métodos Analíticos Espectroscópicos y Electroquímicos (G1041321).
A medium level of English (particularly reading comprehension) is highly recommended to participate in the English group.
Enrolled students need to know and obey the "normas xerais de seguridade nos laboratorios de prácticas" of the University of Santiago de Compostela, to participate in laboratory sessions. This regulation is in the webpage (www.usc.es/estaticos/servizos/sprl/normalumlab.pdf)
Maria Pilar Llompart Vizoso
Coordinador/a- Department
- Analytical Chemistry, Nutrition and Bromatology
- Area
- Analytical Chemistry
- Phone
- 881814225
- maria.llompart [at] usc.es
- Category
- Professor: University Professor
Antonio Moreda Piñeiro
- Department
- Analytical Chemistry, Nutrition and Bromatology
- Area
- Analytical Chemistry
- Phone
- 881814375
- antonio.moreda [at] usc.es
- Category
- Professor: University Professor
Andrés Duque Villaverde
- Department
- Analytical Chemistry, Nutrition and Bromatology
- Area
- Analytical Chemistry
- andres.duque.villaverde [at] usc.es
- Category
- USC Pre-doctoral Contract
| Tuesday | |||
|---|---|---|---|
| 11:00-12:00 | Grupo /CLE_01 | Spanish | Organic Chemistry Classroom (1st floor) |
| Wednesday | |||
| 09:00-10:00 | Grupo /CLE_03 | English | Classroom 3.44 |
| 09:00-10:00 | Grupo /CLE_02 | Spanish | Inorganic Chemistry Classroom (1st floor) |
| 12:00-13:00 | Grupo /CLE_01 | Spanish | Organic Chemistry Classroom (1st floor) |
| Thursday | |||
| 10:00-11:00 | Grupo /CLE_03 | English | Classroom 3.44 |
| 10:00-11:00 | Grupo /CLE_02 | Spanish | Inorganic Chemistry Classroom (1st floor) |
| 11:00-12:00 | Grupo /CLE_01 | Spanish | Organic Chemistry Classroom (1st floor) |
| 05.13.2025 10:00-14:00 | Grupo /CLE_01 | Biology Classroom (3rd floor) |
| 05.13.2025 10:00-14:00 | Grupo /CLE_01 | Mathematics Classroom (3rd floor) |
| 06.30.2025 16:00-20:00 | Grupo /CLE_01 | Inorganic Chemistry Classroom (1st floor) |
| 06.30.2025 16:00-20:00 | Grupo /CLE_01 | Organic Chemistry Classroom (1st floor) |