ECTS credits ECTS credits: 6
ECTS Hours Rules/Memories Hours of tutorials: 3 Expository Class: 30 Interactive Classroom: 18 Total: 51
Use languages Spanish, Galician
Type: Ordinary Degree Subject RD 1393/2007 - 822/2021
Departments: Chemical Physics
Areas: Chemical Physics
Center Faculty of Sciences
Call: First Semester
Teaching: With teaching
Enrolment: Enrollable
- Identify and apply the most important spectroscopic techniques for the study of biomolecules.
- Identify and apply the different microscopic techniques for the study of biomolecules.
Theory:
- Introduction to spectroscopic and microscopic techniques.
- UV-Vis absorption spectroscopy: absorption of proteins and nucleic acids.
- Fluorescence spectroscopy: intrinsic and extrinsic fluorescent probes, fluorescence quantum yield and lifetime, quenching of fluorescence, FRET methods.
- Circular dichroism.
- Infrared and Raman spectroscopies.
- Nuclear Magnetic Resonance of biomolecules.
- Microscopies: transmission, fluorescence, confocal, AFM, high resolution,….
Laboratory program: laboratory experiments for the study of biomolecules using the techniques studied (according to availability).
The essential contents of this subject are included in the activity sheets for each topic that will be available in the virtual classroom. The activity sheets are mainly based on the following books. Another additional bibliography will be indicated in the virtual classroom if necessary.
• P. W. Atkins y J. de Paula, Physical Chemistry for the Life Sciences, Oxford University Press, Oxford, 2011.
• P. W. Atkins, J. de Paula y James Keeler, Atkins’ physical chemistry, 11th edition, Oxford University Press, Oxford, 2017. Spanish version: Atkins Química Física, 8th edition, Editorial Médica Panamericana, Buenos Aires, 2008. Student`s solutions manual for physical chemistry: solutions to problems corresponding the 6th edition.
• J. R. Lakowicz, Principles of Fluorescence Spectroscopy, Springer, 2006.
• E. M. Goldys (ed), Fluorescence Applications in Biothechnology and the Life Sciences, Wiley-Blackwell, 2009.
• M. Sauer, J. Hofkens y J. Enderlein, Handbook of Fluorescence Spectroscopy and Imaging, Wiley-VCH, 2011.
• P. R. Selvin y T. Ha, Single-Molecule Techniques: A Laboratory Manual; Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, 2008.
Knowledge
Con10: Acquire knowledge and distinguish the different applications of the main experimental and instrumentation methods.
Skills
H/D08: Plan and use the main experimental methods aimed at the analysis of biological activity, isolation techniques, characterization of biological molecules, development of therapeutic systems and evaluation of active substances.
Competencies
Comp04: Act professionally, governed by ethical principles, scientific rigor and sustainable development.
Comp08: Be able to communicate conclusions and knowledge in an argued manner to audiences
specialized and non-specialized with clarity and precision, also in foreign languages, mainly in English.
LECTURES:
The methodology of the inverted classroom will be used, where the student works the theoretical concepts asynchronously before the class and solves related exercises and practical cases in groups during the class with the help of the professor. For this, the contents of the subject are divided in activity sheets and put at the disposal of the students in the virtual classroom. Before each class the degree of understanding reached by the students in each one of the concepts object of study will be checked with an on-line questionnaire. Based on the results, the teacher gives a brief explanation at the beginning of the class of those concepts that the students have not well understood. Afterwards the students solve in groups exercises and practical cases in which they apply the theoretical concepts studied.
SEMINARS:
In these classes, the students solve and deliver practical cases. The assessment of the student's work will contribute to the continuous evaluation.
the continuous evaluation.
TUTORIAL CLASSES:
These classes will be used for the presentation of the work carried out in the laboratory lessons.
LABORATORY LESSONS:
The laboratory lessons are experimental work sessions in the laboratory in which the students come into contact with the techniques under study and consolidate the knowledge acquired in the theory classes. Students should attend the practical lessons knowing the tasks to be carried out and the related theoretical concepts, for which they will have the corresponding teaching material in advance.
ATTENDANCE: it is compulsory the assist to the laboratory lessons, seminars and tutorial classes except in case of an exceptional cause which has to be properly justified. The absence to these activities will have to be justified documentarily, accepting reasons of examination and of health, as well as those cases included in the university regulations.
For each unjustified absence from the laboratory lessons, 0.5 points will be subtracted from the final grade of the subject. Each unjustified absence from seminars or tutorials will mean a zero mark in the corresponding deliverable.
ASSESSMENT OF THE STUDENTS:
The qualification “aprobado” will be obtained for a final grade of 5 out of 10. The final grade, both in the first and in the second opportunities, will be determined based on the evaluation of the following aspects:
• 25% of the final grade: continuous evaluation, which takes into account the completion of assignments, exercises, practical cases, and questionnaires during seminars, and lectures.
Evaluated competences: Con10, H/D08, Comp04, Comp08.
• 25% of the final grade: evaluation of the laboratory lessons based on the previous preparation of the students, the laboratory report and the continuous evaluation in the laboratory. It is a requirement to obtain a minimum grade of 4,0 over 10 in the laboratory lessons to pass the subject.
Evaluated competences: Con10, H/D08, Comp04, Comp08.
• 50% of the final grade: evaluation of the final exam of the subject with conceptual questions and problems, complementary to the continuous evaluation and the laboratory grade, both in the first and in the second opportunity and in any of the scenes. It is a requirement to obtain a minimum grade of 4,0 over 10 in the exam to pass the subject.
Evaluated competences: Con10, H/D08, Comp04.
PLAGIARISM AND MISUSE OF TECHNOLOGIES IN THE CONDUCT OF TASKS OR TESTS: "For cases of fraudulent execution of exercises or tests, the provisions of the Regulations for the evaluation of student academic performance and revision of qualifications will apply."
Second Opportunity Assessment:
The same conditions apply as in the first opportunity. The marks of the continuous evaluation and laboratory lessons cannot be recovered, keeping those obtained during the academic period.
Evaluation in second call and later:
The continuous evaluation and exam marks are not saved for subsequent calls, only the laboratory lessons mark is saved if it is greater than or equal to 5.0 out of 10 and for a maximum of two more academic years.
This course involves 51 hours of attendance (plus 4 hours for the exams), so that students will invest about 95 additional hours of individual work, split between the home-study and deepening of the theory and the application of such content to the resolution of practical cases and the preparation of reports.
For each of the classroom activities the following times of personal work are estimated:
- Lectures: 30 h classroom, 30 h of individual personal work or in a group.
- Seminars: 3 h classroom, 17 h of individual personal work or in a group.
- Tutorial classes: 3 h classroom, 37 h of individual personal work or in a group.
- Laboratory lessons: 15 h laboratory, 15 h of individual personal work.
• It is important to keep the study of the subject "up to date" preparing each class based on the corresponding cards. The comprehension questions and some of the short exercises should be done before each class. The student must answer the previous questionnaire of each class indicating the doubts and difficulties with the contents.
• The resolution of practical cases is fundamental for the learning of this subject.
• It is indispensable to prepare the laboratory lessons before entering in the laboratory.
• It is recommended to consult regularly the virtual classroom of the subject, where all the relevant information about the program, schedules and organization of the subject will be available, as well as the activity sheets and complementary audiovisual material (slides, simulations, web links, etc.) and also on-line questionnaires which help to prepare the theoretical contents.
This document has been machine-translated from the Spanish original.
Maria De La Merced Novo Rodriguez
- Department
- Chemical Physics
- Area
- Chemical Physics
- m.novo [at] usc.es
- Category
- Professor: University Professor
Wajih Al-Soufi
Coordinador/a- Department
- Chemical Physics
- Area
- Chemical Physics
- Phone
- 982824114
- wajih.al-soufi [at] usc.es
- Category
- Professor: University Professor
Diego Cora Calvo
- Department
- Chemical Physics
- Area
- Chemical Physics
- diego.cora.calvo [at] usc.es
- Category
- Predoutoral USC_Campus Terra
| Tuesday | |||
|---|---|---|---|
| 13:00-14:00 | Grupo /CLE_01 | Galician | 2P CLASSROOM 5 SECOND FLOOR |
| 01.15.2025 10:00-14:00 | Grupo /CLE_01 | 2P CLASSROOM 2 SECOND FLOOR |
| 01.15.2025 10:00-14:00 | Grupo /CLE_01 | 2P CLASSROOM 5 SECOND FLOOR |
| 06.23.2025 16:00-20:00 | Grupo /CLE_01 | 2P CLASSROOM 2 SECOND FLOOR |
| 06.23.2025 16:00-20:00 | Grupo /CLE_01 | 2P CLASSROOM 5 SECOND FLOOR |